TY - JOUR
T1 - Differential effects of histone deacetylase inhibitors on Interleukin-18 gene expression in myeloid cells
AU - Koyama, Noriko
AU - Koschmieder, Steffen
AU - Tyagi, Sandhya
AU - Nürnberger, Heike
AU - Wagner, Sandra
AU - Hoelzer, Dieter
AU - Gerhard Ottmann, Oliver
AU - Kalina, Uwe
AU - Böcker, U.
PY - 2002
Y1 - 2002
N2 - Histone deacetyrase (HDAC) inhibitors induce growth arrest and differentiation of leukemia cell lines and tumor cells derived from a large variety of human tissues. Here we showed that HDAC inhibitors sodium butyrate, TSA, and valproate regulated the expression of Interleukin-18 (IL-18), a cytokine with antitumor and proinflammatory properties, in human acute myeloid leukemia cell lines U937 and HEL. Sodium butyrate increased expression of IL-18 protein and mRNA and activated 1357bp IL-18 gene promoter construct. IL-18 mRNA level was up-regulated by TSA or valproate, which also activated IL-18 full-length promoter. While sodium butyrate or TSA stimulated the 108-bp IL-18 minimal promoter, valproate failed to activate it, indicating that valproate may use a distinct mechanism from sodium butyrate and TSA to activate IL-18 gene expression.
AB - Histone deacetyrase (HDAC) inhibitors induce growth arrest and differentiation of leukemia cell lines and tumor cells derived from a large variety of human tissues. Here we showed that HDAC inhibitors sodium butyrate, TSA, and valproate regulated the expression of Interleukin-18 (IL-18), a cytokine with antitumor and proinflammatory properties, in human acute myeloid leukemia cell lines U937 and HEL. Sodium butyrate increased expression of IL-18 protein and mRNA and activated 1357bp IL-18 gene promoter construct. IL-18 mRNA level was up-regulated by TSA or valproate, which also activated IL-18 full-length promoter. While sodium butyrate or TSA stimulated the 108-bp IL-18 minimal promoter, valproate failed to activate it, indicating that valproate may use a distinct mechanism from sodium butyrate and TSA to activate IL-18 gene expression.
KW - Differentiation
KW - Histone deacetylase inhibitors
KW - Interleukin-18 promoter
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U2 - 10.1006/bbrc.2002.6753
DO - 10.1006/bbrc.2002.6753
M3 - Article
C2 - 11944905
AN - SCOPUS:0036288816
SN - 0006-291X
VL - 292
SP - 937
EP - 943
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 4
ER -
